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Image Search Results
Journal: The Journal of Experimental Medicine
Article Title: Efficient Generation of a Hepatitis B Virus Cytotoxic T Lymphocyte Epitope Requires the Structural Features of Immunoproteasomes
doi:
Figure Lengend Snippet: Time-dependent generation of a potential precursor peptide HBcAg141-162 and of HBcAg141-151. HBcAg131-162 was digested for 0.5, 1, 2, 4, 8, and 24 h with 20S proteasome isolated from T2 cells and T2 LMP2+7 transfectant cells. Cleavage products were separated by HPLC as described in Materials and Methods but analyzed on-line by an ion trap mass spectrometer (ThermoQuest) equipped with an electrospray ion source, which results in increased sensitivity. Time course of the potential precursor peptide HBcAg141-162, which is generated by a single cleavage after Leu140 (A), and of the antigenic HBcAg141-151 (B) is depicted. Plotted values were confirmed by repeated analysis of the same digests.
Article Snippet: C4 (BALB/c) mouse fibroblast cells were cultured in Iscove's MEM (Biochrom), and the human cervical carcinoma cell line HeLa, the human lymphoblastoid cell line T2 , and
Techniques: Isolation, Transfection, Mass Spectrometry, Generated
Journal: The Journal of Experimental Medicine
Article Title: Efficient Generation of a Hepatitis B Virus Cytotoxic T Lymphocyte Epitope Requires the Structural Features of Immunoproteasomes
doi:
Figure Lengend Snippet: Incorporation of the LMP2 and LMP7 subunits in 20S proteasomes of T2 transfectant lines. 500 ng (A) or 150 ng (B) of purified 20S proteasomes was separated by SDS-PAGE and probed with mouse LMP2–specific (A) or mouse LMP7–specific (B) polyclonal rabbit antisera. Molecular mass markers (kD) are shown at left.
Article Snippet: C4 (BALB/c) mouse fibroblast cells were cultured in Iscove's MEM (Biochrom), and the human cervical carcinoma cell line HeLa, the human lymphoblastoid cell line T2 , and
Techniques: Transfection, Purification, SDS Page
Journal: The Journal of Experimental Medicine
Article Title: Efficient Generation of a Hepatitis B Virus Cytotoxic T Lymphocyte Epitope Requires the Structural Features of Immunoproteasomes
doi:
Figure Lengend Snippet: Kinetic analysis of the production of HBcAg141-151 and of related peptide fragments. HBcAg131-162 was digested for 4, 8, or 24 h with 20S proteasomes isolated from T2 LMP2+7 (A) and T2 LMP2+7 T1A transfectant cells (B) or mock-digested (C). Cleavage products were analyzed as described in Materials and Methods. Relative abundances of 11 selected peptides are plotted. Shown data were reproduced in a second, independent experiment.
Article Snippet: C4 (BALB/c) mouse fibroblast cells were cultured in Iscove's MEM (Biochrom), and the human cervical carcinoma cell line HeLa, the human lymphoblastoid cell line T2 , and
Techniques: Isolation, Transfection